Developmental Biology and Regeneration

Research interest

In contrast to adult mammals, zebrafish have the capacity to regenerate their hearts upon several types of injury. In the laboratory, we use cryoinjury, to induce a cardiac tissue damage with the aim to mimic the consequences of tissue loss upon myocardial infarction González-Rosa and Mercader, 2012). Upon eliminating up to ¼ of the cardiac ventricle zebrafish are able to regenerate the damaged myocardium and recover cardiac function (González-Rosa et al., 2014). Massive collagen depositions precede the process of cardiac regeneration, revealing that, in contrast to mammals, cardiac fibrosis is reversible in the zebrafish and occurs as an intermediate step during regeneration (González-Rosa et al., 2011). We are interested in understanding the mechanisms through with in the zebrafish, the fibrotic tissue, including extracellular matrix and myofibroblast regress, as this might have implications for the design of antifibrotic strategies in mammals. We are also interested in understanding which are the cells contributing to the fibrotic response.

 

Unbenanntes Dokument

CNIC Konferenz 2016

Mechanical forces in physiology and disease.
Webseite - Poster

 

The epicardium, the outer layer covering the myocardium contains precursors contributing to the fibrotic response observed after cryoinjuring the zebrafish heart (González-Rosa et al., 2012). We are interested in analysing the mechanisms of epicardium reestablishment as well as the influence of this layer during the regeneration process.

We are also interested in analysing how the epicardium forms during embryonic development. The epicardium derived from the proepicardium, a cell cluster emerging at the inflow region of the forming heart tube (Peralta et al., 2014). Using live imaging in zebrafish embryos we are studying the mechanisms through which proepicardial cells are emerging from the pericardial wall and attach to the myocardium. We found that proepicardial cells detach from the pericardial wall and are advected around the cardiac ventricle, and subsequently attach to its surface (Peralta et al., 2013). We also found that this process is dependent on the heartbeat. Our current effort is dedicated to understand the underlying mechanosensory pathways as well as the role of extracellular and secreted molecules controlling proepicardium and epicardium formation.

Unbenanntes Dokument
Model systems for regeneration: zebrafish.
Marques et al., 2019
Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish
Sanz-Morejón A et al., 2019
Pre-existent adult sox10+ cardiomyocytes contribute to myocardial regeneration in the zebrafish.
Marcos Sande-Melón et al., 2019
Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells.
Andrés-Delgado L, Ernst A, et al., 2019
Transient fibrosis resolves via fibroblast inactivation in the regenerating zebrafish heart.
Sánchez-Iranzo et al., 2018
Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration.
Sánchez-Iranzo et al., 2018
Interplay between cardiac function and heart development.
Andrés-Delgado & Mercader, 2016
Use of Echocardiography Reveals Reestablishment of Ventricular Pumping Efficiency and Partial Ventricular Wall Motion Recovery upon Ventricular Cryoinjury in the Zebrafish.
González-Rosa et al., 2014
Heartbeat-Driven Pericardiac Fluid Forces Contribute to Epicardium Morphogenesis.
Peralta et al., 2013
Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration.
González-Rosa et al., 2012
Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish.
González-Rosa et al., 2012
Extensive scar formation and regression during heart regeneration after cryoinjury in zebrafish.
González-Rosa et al., 2011
Unbenanntes Dokument
Unbenanntes Dokument
 
Intraflagellar transport complex B proteins regulate the Hippo effector Yap1 during cardiogenesis.
Peralta et al., 2019
 
Neuropilin 1 mediates epicardial activation and revascularization in the regenerating zebrafish heart.
Lowe V, Wisniewski L, Sayers J, Evans I, Frankel P, Mercader-Huber N, Zachary IC, Pellet-Many C,, 2019
 
Retinal microglia signaling affects Müller cell behavior in the zebrafish following laser injury induction.
Conedera FM, Pousa AMQ, Mercader N, Tschopp M, Enzmann V., 2019
Elly Tanaka’s passion for exploring animal regeneration.
Mercader N. & Serras F., 2018
Can broken hearts be mended? Ken Poss, a pioneer on heart regeneration research.
Mercader N. & Serras F., 2018
Models to crack the code of organ regeneration.
Mercader N. & Serras F., 2018
 
High-throughput identification of small molecules that affect human embryonic vascular development.
Vazão et al., 2017
 
A structural variant in the 5'-flanking region of the TWIST2 gene affects melanocyte development in belted cattle.
Awashti et al., 2017
Analysis of the dynamic co-expression network of heart regeneration in the zebrafish.
Cogliati S. et al, , 2016
Analysis of the dynamic co-expression network of heart regeneration in the zebrafish.
Rodius S. et al., 2016
2C-Cas9: a versatile tool for clonal analysis of gene function.
Di Donato et al., 2016
Proepicardial cells go for a swim: how fluid flows guide epicardial progenitors to the heart.
Mercader N. et al., 2013
Heart regeneration: dream or reality?
Mercader N. et al., 2013
Unbenanntes Dokument
Unbenanntes Dokument
ERC Consolidator Grant 2018 TransReg
Schweizerischer Nationalfonds (SNF)

We are seeking to recruit a Bioinformatician with expertise in transcriptomics. Please contact nadia.mercader@ana.unibe.ch for further details.

We are currently looking for enthusiastic undegraduate students interested in performing their Master in science project on cardiac development or heart regeneration in the zebrafish.

Please write an email including a motivation letter and CV to nadia.mercader@ana.unibe.ch.