Stereology Workshop

Workshop on Quantitative Microscopy 2017

Stereology Week: August 14th to 18th 2017
Hands-on VISIOPHARM newCAST: August 19th and 20th 2017

Stereology provides biomedical researchers with efficient and unbiased tools to obtain real 3D data like volume, surface, length, and number of organs, tissues, cells, and organelles from measurements done on microscopic sections.

If this is important for your research, this workshop is for you!

In collaboration with the Microscopy Imaging Center (MIC), University of Bern

Scope

This workshop provides an introduction to state-of-the-art stereology with emphasis on its practical application in biomedicine. Experimental design, sampling, use of stereological probes, estimation of stereological parameters, and the application of statistical methods appropriate for stereology are covered. The workshop encompasses lectures, classroom exercises with real biomedical images, and laboratory practicals demonstrating the implementation of proper sampling and processing techniques on real biological organs.

This workshop is intended for students and researchers from the field of biology and medicine interested in sampling and measurement of 3D structures. Applications involving any kind of microscopy (e.g. light microscopy, confocal laser scanning microscopy, multi-photon microscopy, electron microscopy) as well as non-invasive scanning techniques like CT and NMR are welcome.

Participants should bring their specific projects and questions. During the workshop, there will be ample opportunity for discussions with the instructors.

Registration

Week: Stereology

Monday:

Presentation of projects by participants
Lecture: Definition of probes, sampling and basic statistics

Lecture: Volume estimation, Cavalieri principle
Exercise: Volume estimation
Practical: Agar embedding and slicing of organs

Tuesday:

Lecture: The fractionator principle (systematic sampling)
Exercise: The fractionator
Lecture: The disector, counting and sampling in 3D
Exercise: Number estimation

Lecture: More disector sampling, including connectivity
Exercise: Connectivity
Exercise: Alveolar number

Wednesday:

Lecture: Length and Surface, IUR
Exercise: Length estimation
Lecture: Ratios, multilevel sampling design, reference trap

Lecture: Vertical sections
Exercise: Surface from vertical sections
Practical: Fractionator sampling, vertical sections; embedding, sectioning and staining

Thursday:

Lecture: Sampling and sizing of particles
Exercise: The Nucleator
Exercise: Point sampled intercepts

Lecture: Tissue deformation, slicing, tools and gadgets
Demonstration: Stereology and imaging machinery: newCAST
Demonstration: Stereology web-tool: the STEPanizer

Friday:

Lecture: All the estimators of particle size
Lunch break
Final presentation of projects by participants

Weekend

Hands-on computer based stereology and imaging machinery: newCAST (Visiopharm, DK)

Saturday
(start 09:00):

Lectures: Introduction to stereology and newCAST, incl. volume estimation, Cavalieri principle
Exercises: Cavalieri volume, fractional volume

Lectures: Number estimation disector (physical and optical), surface area estimation, and length estimation
Exercises: Physical disector, optical disector, surface area, local volumes using nucleator / rotator

Sunday
(end 17:00):

Exercises: Microscop setup, calibration, image acquisition
Exercises: Optical disector

Lectures: Automation in stereology software, new sampling techniques (Proportionator)
Exercises: Microimager, Auto disector, Point Sampled Intercept

Dr. Johnnie Bremholm Andersen

Visiopharm A/S, Hoersholm, Denmark
e-mail

Prof. Heinz Fehrenbach

Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften (FZB), Borstel, Germany
e-mail

Beat Haenni, head of laboratory

Institute of Anatomy, Experimental Morphology, University of Bern, Switzerland
e-mail

PhD Christina Brandenberger

Institute for Functional and Applied Anatomy, Hannover Medical School, Germany
e-mail

Prof. Jens R. Nyengaard

Stereology and EM Laboratory, University of Aarhus, Denmark
e-mail

Prof. Matthias Ochs

Institute for Functional and Applied Anatomy, Hannover Medical School, Germany
e-mail

Dr. Stefan Tschanz (Organizer)

Institute of Anatomy, IT- Department/Stereology, University of Bern, Switzerland
e-mail

Stefan Tschanz, Dr. med., Software Eng.

Institute of Anatomy, Baltzerstrasse 2, CH-3000 Bern 9
Tel. +41 (0)31 631 84 78
Fax +41 (0)31 631 38 07
e-mail

Institute of Anatomy. Baltzerstrasse 2, 3000 Bern 9, Switzerland

Entrance of the old building, Bühlstrasse 26
Lesesaal first floor (lectures), Room B 212 OPS (lab. demos)

Map